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Thereafter, DTT and EDTA were removed by passing the sample through a PD-10 column (Cytiva) pre-equilibrated with Chelex-treated and degassed reaction buffer (50 mM HEPES-NaOH, pH 7.2, and 150 mM NaCl)
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Vibrant America provided funding for this study in the form of salaries for authors (HK, IR, MP, VJ, KK, TW, KB, and JR)
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