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The supernatant and mitochondrial samples were neutralized with 3 M KOH/0.1 mM Tris and stored at -72°C until used for determination of metabolites by standard enzymatic methods (26)
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We hence explored the molecular changes induced by slowing down mitochondrial translation, and uncovered that a conserved program for mitochondrial-cytosolic translational balance is activated upon repression of mitochondrial translation 16
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