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6 Challenges and outlook 6.1 Limitations of current studies Although substantial progress has been made in recent years in elucidating the role of epithelial-to-mesenchymal transition (EMT) in the pathogenesis of pulmonary fibrosis, the field is still hampered by numerous controversies and technical bottlenecks that require systematic investigation and methodological innovation
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The SYBR green channel was used to detect fluorescence as the wavelength of flavin fluorescence overlaps with that of SYBR green
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