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Ten μl each was taken from the mixture 0.25, 3, 10, 30 min after the application of Na 2 S 2 or Na 2 S 3 , and labeled with 1 mM monobromobimane for 20 min at room temperature, and then acetic acid was added to the final concentration of 1% and incubated for 15 min on ice
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The conical tubes were centrifuged (3000 × g , 22 °C, 5 min) and the resulting clarified supernatant transferred to clean 8- or 20-mL glass vials which were concentrated in vacuo and suspended in methanol as described for endo -metabolome samples
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