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In vitro succination reaction GSH (2 mM) and 8 mM 13 C 2 -fumarate were mixed in 2 ml of a solution composed of 50 mM NaCl, 20 mM Tris and 0.1% Triton-X-100, pH=7.4, 4 °C for 8 h in the presence or absence of cell lysate obtained from 2 × 10 6 Fh1 Δ/Δ cells previously heat inactivated (100 °C for 1 h) or left untreated

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