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It possesses the ability to recognize and bind to the fungal cell wall-associated D-mannosidase and form a ternary complex consisting of pradimicin, D-mannose, and calcium [150]

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尺寸:1千克（2.2磅）

The lysate was centrifuged at 140,000 g for 15 min in a Ti45 rotor (Beckman Colter) and incubated with 2 mL of affinity Glutathione Sepharose resin (GE Healthcare 17-0756-05), washed with 100 mL of wash buffer containing 20 mM HEPES pH 8.0, 300 mM NaCl and 1 mM TCEP and 100 mL of TEV buffer containing 20 mM HEPES pH 8.0, 200 mM NaCl and 1 mM TCEP

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