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Reduced GSH levels were obtained by differences between total GSH and GSSG and normalized to protein content of each sample determined by Bradford method (Bio-Rad, Hercules, CA, USA)

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Alternative quantitative approaches include second harmonic generation (SHG) and two-photon excited fluorescence microscopy, which enable label-free detection of fibrillar collagen and have been validated against histological staining and biochemica Apoptosis Apoptosis, also called programmed cell death, is generally characterized by distinct morphological characteristics