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A benefit of using biotin as an The affinity tag is that it has little effect on due to its small size, the activity of a large biomolecule (244Da).Streptavidin is a large protein (60 kDa) that can be obtained from Streptomyces avidinii and have an affinity constant of 1013 M–1 for biotin

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These pharmacokinetic features, characterized by rapid clearance and short systemic exposure, further challenge their preclinical-to-clinical applicability