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In vivo half-life studies In hemizygous human FcRn Tg32 mice, groups of male mice (7–8 weeks, 21.5–30.5 g, 4–5 mice/cage/group) were given IV injection, into the lateral tail-vein, of 1 mg/kg of unfused WT albumin and QMP albumin variants, diluted in DPBS (pH 7.4) (Sigma-Aldrich)

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Subsequent introduction of two random mutations, Y444A and G484A, to D433N EcGGT variant further enhanced catalytic efficiency for GL-7-ACA substrate by 50-fold (Yamada et al., 2008)